ANALYSIS OF TELOMERIC SEQUENCES IN THE PROGENY OF BLEOMYCIN-EXPOSED CELLS

PAVIOLO N; VIDAL BRAVO M; BOLZÁN A

San Juan

Encuentro; SECOND JOINT MEETING OF THE BIOLOGY SOCIETIES FROM ARGENTINA; 2011

Sociedad Argentina de Biología, Sociedad de Biología de Cuyo y Sociedad de Biología de Córdoba

The chromosome damage and its relationship with the telomeric sequences was analyzed in the progeny of mammalian cells exposed to the antitumoral antibiotic bleomycin (BLM). The aim ofthe present work was to evaluate if chromosome damage induced at telomeres and interstitial telomeric sequences (ITS) persisted on the progeny of the cell population originally exposed to the mutagen. Fluorescence in situ hybridization (FISH) with a PNA telomeric probe was applied on chromosome preparations of Chinese Hamster Ovary cells (CHO cell line) and rat cells derived from adipose tissue (ADIPO-P2 cell line). Cells were exposed in the log phase of growth to 2.5 μg/ml of BLM during 30 minutes at 37°C. Cytogenetic analysis revealed a significant increase in the frequency of chromosomal aberrations after 18 h after treatment in CHO cells treated with BLM compared with unexposed cells. BLM also induced a significant increase in the frequency of chromosomal aberrations 6-10 days after treatment on both cell lines. Our results also indicated that BLM induced delayed chromosomal instability, revealed by amplification of ITS in CHO cells and the presence of incomplete chromosomes in ADIPO-P2 cells