The inflammatory profile of circulating monocytes is altered in patients with Mood Disorder

ARENA AR; OLAVIAGA, ALEJANDRO; ZOTELO ULFELDT, TAMARA; CARRERA SILVA, EUGENIO A.; PENNA, MELINA B.; HUNTER, FERNANDO; ARMESTO, ARNALDO R.; ERRASTI, ANDREA E.; GRENDAS, LEANDRO N.; MAGRINI, FRANCO; RODRIGUEZ, M. BELÉN; DARAY, FEDERICO M.

Buenos Aires

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA SAIC SAI SAFIS 2020; 2020

SAIC SAI SAFIS

Mood Disorders (MD) affects over 350 million people around the world. Despite the public impact of MD, its etiology remains unknown. In recent years, evidence reveals a key role of the immune system in the development and maintenance of MD. We have recently shown that plasma proinflammatory IL-12 and IL-6 were increased in patients with depression and suicidal behavior. Interestingly, these cytokines positively correlate with altered monocytes activation and proportions.We aim to determine the differences in the profile and activation status of circulating monocytes in patients with MD vs healthy controls (HC) at baseline and after a 6-month follow-up. Psychiatrists used the International Psychiatry Interview (MINI) to diagnose MD, and the Hamilton Depression Rating Scale (HADRS) to define active disease (AD) or non-active disease (NAD). Blood samples were obtained, and the classical, intermediate, and non-classical monocyte subsets were analyzed by FACS. After 6-month, patients were re-evaluated for depressive and inflammatory status. The study was approved by IRB, each participant gives written consent.MD sample was 23% male, 77% female with a 25-62 year age range. The percentage of classical monocytes was reduced in MD (72.0 ± 2.6, N=23) vs HC (87.0 ± 1.7, N=5) together with an increase of the intermediate fraction, MD (11.8 ± 1.6) vs HC (4.4 ± 0.5). After segregation, the AD patients showed the most significant change in terms of percentage of classical (p