INFLUENCE OF THE INTESTINAL ENVIRONMENT ON ESCHERICHIA COLI O157:H7 PATHOGENICITY AND INFECTIVITY

BERNAL, ALAN; RAMOS, MARIA VICTORIA; PALERMO, MARINA SANDRA; GIRARDI, JI; RUMBO, MARTIN; FERNANDEZ BRANDO, ROMINA; ERREA, AGUSTINA

Buenos Aires

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020 LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC) LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS); 2020

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC), SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI), SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS)

We previously demonstrated a different outcome post E. coliO157:H7 (O157) infection between weaned C57BL and BALB mice.Although bacterial burden is similar, C57BL show increased tissuedamage and mortality. The aim of this work is to investigate howintestinal environment affects pathogen virulence and horizontaltransmission in both strains.3 days post infection (pi), we analyzed the Stx production of recovered O157 from C57BL and BALB feces. Results showed similar Stxproduction by VERO and ELISA assays. Besides, we determinedthe inflammatory activity of feces through a LPS-dependent HekBlue-hTLR4 reporter line assay. Feces from C57BL showed inflammatory activity at day 1, 2 and 3 pi (p