Different regulation of circulating and renal cytokines in Shiga toxin treated IL10 deficient mice

MONGELOS, MICAELA; ISTURIZ, MARTIN AMADEO; PALERMO, MARINA SANDRA; BRUBALLA, ANDREA; REARTE, BARBARA; GONZALO PINEDA,; ALBA SOTO, CATALINA; TODERO, FLORENCIA; ZOTTA, ELSA; RAMOS, MARIA V.

Mar del Plata, Prov Buenos Aires, Republica Argentina

Congreso; SAIC - SAI - SAFIS 2018; 2018

Hemolytic Uremic Syndrome is a disease triggered by Shiga toxin (Stx)characterized by hemolytic anemia, thrombocytopenia and renal failure. Althoughthe role of inflammatory factors in this disease is well documented, the role of antiinflammatorycytokines, such as IL-10, is less clear. Previously we demonstratedthat IL-10 deficient mice (IL-10-/-) showed a higher survival after Stx2 inoculation,associated with lower renal damage reflected by reduced plasma creatinine levels.In this work, we studied the profile of pro- and anti-inflammatory factors in bloodand kidney.Before or 3h after administration of 1LD100 of Stx2 e.v., IL-10-/- and Wt mice wereeuthanized and plasmas were collected. The kidneys were excised and the tissueshomogenized in HEPES buffer. The plasma (pg/ml) and renal levels (pg/mg totalprotein) of IL-10, Corticosterone, IL-6 and TNF-α were quantified by ELISA.Even though IL-10 was not detected in Wt plasma, before and after Stx2, renallevel of this cytokine was higher before than after Stx2 inoculation(Wt0h:57.31±8.16/WT3h:26.72±4.47*,*