THE INTERLEUKIN-4/SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 6 AXIS ENHANCES THE LIPOLYTIC ACTIVITY PREVENTING THE FORMATION OF FOAMY MACROPHAGES INDUCED BY TUBERCULOUS PLEURAL EFFUSIONS

MARÍN FRANCO, JOSÉ LUIS; POGGI, SUSANA; MARIDONNEAU-PARINI, ISABELLE; GENOULA, MELANIE; MORAÑA, EDUARDO JOSE; NEYROLLES, OLIVIER; BALBOA, LUCIANA; FERREYRA, MALENA; PALMERO, DOMINGO; SASIAIN, MARIA DEL CARMEN

Mar del Plata

Congreso; Reunión Conjunta SAIC SAI SAFIS 2018; 2018

The ability of Mycobacterium tuberculosis (Mtb) to persist inside the host relies on its numerous immune evasion strategies such as the dysregulation of the lipid metabolism leading to foamy macrophages (FMs). FMs are cells filled of lipids which fail to control the infection. Recently, we showed that the tuberculous pleural effusions (TB-PE) promote the formation of FMs in uninfected-macrophages leading to an immunosuppressive profile though the activation of the IL-10/STAT-3 axis. Herein, we wondered whether different activation programs in human macrophages differ in their ability to form FMs after the treatment with TB-PE. For that, we polarized macrophages with either IL-4, IL-10 or IFN-y, treated them or not with TB-PE, and evaluated the intracellular accumulation of lipid bodies (LBs). We observed that M(IL-4) did not accumulate LBs under TB-PE treatment in comparison with unpolarized macrophages (M0), M(IFN-γ) or M(IL-10) (p