gamma/delta T lymphocytes modulate neutrophil function

SHIROMIZU, CAROLINA MAIUMI; KEITELMAN, IRENE A; JANCIC, CAROLINA C.; CHIAPPETTA, CAMILA; TOWSTYKA, NADIA; ANALÍA, TREVANI S.; ROSO, DAVID; SABBIONE, FLORENCIA

Mar del Plata

Congreso; 66 Reunión Anual de la Sociedad Argentina de Inmunología; 2018

SAI - SAIC

We have previously reported that neutrophils acquired different profiles of activation when they are cultured in presence of γδ T cells activated by phosphoantigens or through CD3. Now, we aim to investigate the mechanism involved in the modulation of neutro¬phils activity by γδ T lymphocytes. γδ T cells were purified from hu¬man peripheral blood mononuclear cells by using an anti-TCR γδ MicroBead isolation kit, and neutrophils were isolated by dextran sedimentation. After purification, γδ T cells were stimulated with an¬ti-CD3 antibodies (250 ng/ml) or phosphoantigens (HMBPP, 10 μM) and cultured with or without neutrophils. After that, we analyzed in γδ T cells, IFN-γ and TNF-α production by ELISA, and CD69 ex¬pression by flow cytometry. Also, we analyzed the activation state of neutrophils and the expression of perforin by flow cytometry. Treat¬ment of γδ T cells with HMBPP or anti-CD3 antibodies increased the expression of CD69 and the production of IFN-γ and TNF-α (p