Deleterious effects of activated neutrophils and NETs on trophoblast cell function in a model of maternal fetal interaction.

G CALO, F. SABBIONE, N. PASCUALI, I KEITELMAN, D VOTA, D PAPARINI, R RAMHORST, F PARBORELL, A TREVANI AND C PÉREZ LEIROS.

Mar del Plata

Congreso; Reunión Anual Conjunta SAI-SAIC-SAFIS; 2018

SAI-SAIC-SAFIS

Trophoblast cells (Tb) interact with different maternal immune cell populations at early pregnancy promoting an anti-inflammatory and tolerogenic response. Uterine vasculature remodeling occurs to accommodate the increasing demand of the growing fetus. Circulating neutrophils are found activated during pregnancy and even more during pathological pregnancies. Vasoactive Intestinal Peptide (VIP) is a pleiotropic peptide with immunomodulatory effects through its action on VPAC1 and VPAC2 receptors, both found in neutrophils.We have already shown that VIP and conditioned media (CM) from human first trimester Tb (Swan-71 cell line) inhibit PMA-induced neutrophil extracellular trap(NET) formation, promote neutrophil apoptosis and revert the anti-apoptotic effect of LPS.Our aim was to evaluate the effect of VIP and trophoblast derived factors on neutrophils and how these conditioned cells could impact likewise on Tb function and profile.Neutrophils were isolated from healthy donors blood. We found that both neutrophils and monosodium urate crystals (MSU)-induced NETs increased the expression of trophoblast IL-8 and decreased TGF-β explored by RT-qPCR (P