Role of alpha-glucans in respiratory burst-induced dendritic cell maturation and function

LAURA CORREA FEO; ROMERO MERCEDES; ALEJANDRA DUARTE; ALEMAN MECEDES

Congreso; 15th International Symposium on Dendritic Cells; 2018

Tuberculosis remains the single largest infectious disease with two million deaths estimated to occur yearly. The spread of Mycobacterium tuberculosis (Mtb) from the lungs to other sites occur before the development of adaptive immune responses. The major carbohydrate constituents from Mtb surface are α-glucans, a sugar which has been implicated in fungal virulence, and represent up to 80% of the extracellular polysaccharides in Mycobacteria. Previous results demonstrated that reactive oxygen species (ROS) in neutrophils are generated by non-opsonized mycobacteria through α-glucans and that the lack of -glucans in Mtb leads to the loss of their ability to induce ROS and also dendritic cell (DC) maturation and antigen presentation. Here we evaluated the role of α-glucans in the entrance of bacteria in DC and the endogenous production of ROS as effectors of the maturation of DC. Results Monocyte-derived DC were incubated 24 hours with LPS and thereafter were treated with Mtb (H37Rv) subjected or not to-amyloglucosidase treatment (Mtb-e) for 15 min with or without H2O2. Thereafter maturation markers were evaluated by cytometry. Expression of CD86 (p