ANGIOGENIC ACTIVITY OF LATE OUTGROWTH ENDOTHELIAL PROGENITOR CELLS IS INHIBITED BY COXSACKIEVIRUS B3

SCHATTNER M; GOMEZ RM; ZUBIRY PR; NEGROTTO S; MENA HA

Buenos Aires

Congreso; Reunion Conjunta de Sociedades Biomédicas; 2017

Coxsackievirus B3 (CVB3) is a frequent etiological agent of human viral myocarditis, characterized by myocardial inflammation, necrosis and subsequent fibrosis. Local or homed stem and progenitor cells are involved in myocardial repair, especially endothelial progenitor cells which have a lead role in new blood vessel formation and tissue revascularization. Here, we aimed to study the effect of CVB3 on the survival and angiogenic activity of late outgrowth endothelial colony forming cells (ECFC).Cord blood-derived CD34+ cells were seeded in EGM2 medium and ECFC colonies were obtained after 14-21 days. ECFC were incubated with CVB3 at a multiplicity of infection (MOI) 0.1 or 1 for 1 hour at 37°C and then exhaustively washed to ensure virus removal. Cell death (ethidium bromide-acridine orange staining), proliferation (cell count), static adhesion to matrix components (cell count), tubule formation (matrigel) and cell adhesion molecule expression (flow cytometry) were analyzed in vitro. Results are expressed as mean ± SEM. Significant differences (*p