Effects of SAgs of the EGC operon on innate immune cells

SARRATEA, MARÍA BELÉN; TOWSTYKA, NADIA YASMÍN; MALCHIODI, EMILIO LUIS; IANNANTUONO LOPEZ, LAURA; FERNÁNDEZ, MARISA MARIEL; NOLI TRUANT, SOFIA; ANTONOGLOU, BELÉN; JANCIC, CAROLINA C

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Bacterial superantigens (SAgs) are enterotoxins that bind to MHCII and TCR molecules, activating as much as 20% of T cells and promoting a cytokine storm which enhances endotoxic shock and produces immunosuppression, hindering the immune response against bacterial infection. The egc operon reported in autochthonous S. aureus strains includes natural variants of the SAgs genesseg, sei, sem, sen and seo. We have previously characterized the complementary activity of SEG, SEI, SEO and SEM on phagocytic cells, and reported a M1 response tendency. In the present work, we investigate if other cells of the innate immunity are responsible for this pro inflammatory profile. Thus, weevaluated the SAgs effect on human purified gamma/delta T cells from healthy donors by flow cytometry and ELISA. We found that, in contrast to the effect on αβ T cells, SAgs activated gamma/delta T cells from 0.1 μM (p