THE ROLE OF IL-10 IN FOAMY MACROPHAGE DIFFERENTATION IN TUBERCULOSIS

MELANIE GENOULA; JOSÉ LUIS MARÍN FRANCO; DENISE KVIATCOVSKY; AYELEN MILILLO; DOMINGO PALMERO; PAULA BARRIONUEVO; ADRIANA FONTANALS; CHRISTEL VEROLLET; MARIA DEL CARMEN SASIAIN; GEANNCARLO LUGO-VILLARINO; MAEVA DUPONT; EDUARDO MORAÑA; BARBARA REARTE; ROGELIO HERNANDEZ-PANDO; LUCIANA BALBOA

Buenos Aires

Congreso; Reunión Sociedad Argentina de Inmunología; 2017

The ability of Mycobacterium tuberculosis (Mtb) to persist relies on its numerous immune evasion strategies such as the dysregulation of the lipid metabolism leading to foamy macrophages (FM). Although FM provide an environment which is essential for bacilli to escape from host immune system, the specific host factors leading to FM induction are unknown. We aimed to characterize which host factors may contribute to FM differentiation in Mtb infection. For that we use the acellular fraction of tuberculous pleural effusions (PE) as a source of local factors released during Mtb infection. We treated human macrophages with PE and evaluated the accumulation of lipid bodies by oil O red staining, cytokines by ELISA, ACAT and pSTAT-3/STAT-3 by western blot, cholesterol by enzymatic assays, CD36 and CD210 by FACS, and bacillary loads by colony-forming units assays. PE induced FM differentiation as observed by the increased in lipid bodies(p