Role of alpha-glucans in the respiratory burst in monocyte-derived dendritic cells

ALEJANDRA DUARTE; ISABEL PIAZZÓN; MARÍA MERCEDES ROMERO; MERCEDES ALEMAN ; LAURA CORREA FEO

Mar del Plata

Congreso; Reunion conjunta SAI-SAIC-SAFE; 2016

Tuberculosis remains the single largest infectious disease with two million deaths estimated to occur yearly, more than any time in history. The spread of Mycobacterium tuberculosis (Mtb) from the lungs to other sites occur before the development of adaptive immune responses. Mtb has evolved successful strategies to invade and persist within host cells. The major carbohydrate constituents from Mtb surface are α-glucans, a sugar which has been implicated in fungal virulence, and represent up to 80% of the extracellular polysaccharides in Mycobacteria. Previous results demonstrated that reactive oxygen species (ROS) in neutrophils are generated by non-opsonized mycobacteria through α-glucans. Here we evaluated the role of α-glucans in the endogenous production of ROS and their impact on the maturation of dendritic cells (DC) and Mtb-specific lymphocytes expansion. Results Monocyte-derived DC were incubated 24 hours with Mtb (H37Rv) subjected or not to alpha-amyloglucosidase treatment (Mtb-e) and thereafter maturation markers were evaluated by cytometry. Expression of CD86 were induced by Mtb (p