Myeloid-derived suppressor cell generation is reduced by All-trans-Retinoic Acid in Lipopolysaccharide-immunosuppressed mice by decreasing proliferation of hematopoietic precursor cells

RODRIGUEZ-RODRIGUES N; DE CAMPOS-NEBEL M; MARTIRE-GRECO D; ISTURIZ M.A; VECCHIONE MB; MEISS R; FERNÁNDEZ G C; CASTILLO LUIS; CORDOBA MORENO M; LANDONI V.I

Mar del Plata

Congreso; Congreso SAI/SAIC 2016; 2016

SAIC/SAI

All-trans-retinoic acid (ATRA) is a derivative of vitamin A with anti-proliferative properties. Immunosuppression by lipopolysaccharide (LPS) inoculation stimulates myelopoiesis with an expansion of myeloid-derived suppressor cells (MDSC). Our aim was to investigate if ATRA was able to modulate MDSC generation by regulating myelopoiesis in murine hematopoietic organs: spleen (spl) and bone marrow (BM). LPS-induced immunosuppression was developed by subcutaneous inoculations of increasing doses of LPS (IS) and ATRA intraperitoneal (IS+A).We found that ATRA decreased the number of CD34+ precursor cells, increased in IS mice (number of CD34+cells (x105) in spl, Control:7,1±1,5; IS:20,1±6,2*; IS+A:8,1±1,2# and in BM Control:5,3±0,1; IS:14,3±2,7*; IS+A:6,1±0,5#,*vs. Control and #vs. IS p