Effect of urate monosodium crystals on gamma delta T cells

TOWSTYKA, NADIA; SABBIONE, FLORENCIA; GEFFNER, JORGE; TREVANI, ANALIA; JANCIC, CAROLINA

Buenos Aires

Congreso; Congreso Internacional SAI-FAIC; 2015

Effect of urate monosodium crystals on gamma delta T cells Nadia Towstyka1 , Florencia Sabbione1 , Jorge Geffner2 , Analia Trevani1 , Carolina Jancic1 1 Instituto de Medicina Experimental (IMEX) - CONICET - Academia Nacional de Medicina, Argentina 2 Instituto de Investigaciones Biomedicas en Retrovirus y SIDA (INBIRS) - CONICET - UBA, Argentina Abstract: Background: Uric acid is the final product of purine metabolism in humans and higher primates. It is released from dying cells and function as an adjuvant that promotes the generation of adaptive immune responses. The inflammatory effect of uric acid depends on its precipitation into monosodium urate (MSU) crystals. MSU crystals could be recognized by dendritic cells, macrophages, and neutrophils. Moreover, antigenpresenting cells can sense uric acid as a proinflammatory endogenous signal released by damaged cells/tissues. Gamma/delta T cells constitute a specialized subset of T lymphocytes, which act as early sensors of cellular stress and infection. We hypothesized that uric acid released from dying cells might function as a proinflammatory danger signal on gamma/delta T cells. Then, neutrophils located in injured tissues could influence gamma/delta T cell functions. Methods: gamma/delta T cells were purified from human peripheral blood by using anti-TCR gamma/delta MicroBead isolation kit, and neutrophils by dextran sedimentation. Purified gamma/delta T cells were incubated with MSU crystals (12.5-300 ug/ml) with or without neutrophils. After 48 hours, we analyzed cytokines production in supernatants by ELISA. Results: MSU crystals induced an increase in TNF-alfa, IFN-gamma, IL-6 and IL-8 production (p