Cholinergic modulation of polymorphonuclear and monocytes cells activation

MOVERER, LUCIANA; GORI, MARÍA SOLEDAD; VERMEULEN, MÓNICA; SCORDO, WALTER; JANCIC, CAROLINA; SALAMONE, GABRIELA

Buenos Aires

Congreso; 63 Reunión Anual de la Sociedad Argentina de Inmunología. 2da FAIC; 2015

Sociedad Argentina de Inmunología - LASID

Abstract: Acetylcholine is an endogenous neurotransmitter ofboth the peripheral and central nervous systems where it is synthesised,stored, and released from cholinergic nerve terminals. However, there is nowincreasing evidence for extraneuronal acetylcholine signalling, which has beenreferred to as a ?non-neuronal cholinergic system?. The neutrophils andmonocytes are important cellular effectors associated with manypathofhysiologies and their cholinergic anti-inflammatory pathway is apotencial target to interrupt several diseases. Previously we describe thepresence of muscarinic receptors (mAchR), choline acetyltransferase (ChAT) andacetylcholinesterase (AChE) in human myeloid dendritic cell (DC) and we alsoshown their cholinergic modulation. To further complement our results the aimof this work is to analyze the cholinergic modulation of polymorphonuclear(PMN) and monocytes (Mo) cells. Methods:The PMN and Mo were isoleted from peripheral blood obtained from healthynonsmoker. The PMN were cultured with or without Ach (10-7 to 10-10M) or FMLP10-3M. Production of cytokines was determined by ELISA and the molecularexpression by FACs. Results: We donot find differences in the release of IL-8 and TNF-alpha in monocytes cells,but we found significant differences when the cells were treated with carbachol10-8M (p<0.05). On the other handwe observed an increase in the expression of CD11b protein when the cells werestimulated with acetylcholine respect to untreated control (p<0.05) Conclusions: The cholinergic system stimulates the cytokinesproduction in Mo and therefore PMN activation by increasing CD11b protein.