Immunological changes in IL-10-deficient mice during the course of immunosuppression induced by bacterial endotoxins.

CÓRDOBA MORENO, MARLINA; FONTANALS, ADRIANA; MARTIRE-GRECO, DAIANA; FERNÁNDEZ, GABRIELA C; ISTURIZ, MARTIN AMADEO; RAMOS, MARIA VICTORIA; REARTE, BARBARA

Buenos Aires

Congreso; IV LASID Meeting. LXIII Argentinean Immunology Society Meeting; 2015

Immunological changes in IL-10-deficient mice during the course of immunosuppression induced by bacterial endotoxins.Córdoba Moreno, Marlina1; Fontanals, Adriana2; Martire-Greco, Daiana1; Fernández, Gabriela C. 1; Isturiz, Martín A.1; Ramos M Victoria1, Rearte, Bárbara11IMEX-CONICET-Academia Nacional de Medicina, 2Fundación Instituto Leloir, Buenos Aires, Argentina.Introduction: In Sepsis caused by Gram negative, the membrane lipopolysaccharides (LPS) of these bacteria -also called endotoxins- induce a severe immunosuppression, the principal cause of death. We previously demonstrated the glucocorticoids participation in this immunosuppression. Objective: The aim of this study was to evaluate the IL-10 contribution to the immunosuppression and its relationship with glucocorticoids. Methods: Wild type (WT) and IL-10 deficient (KO) BALB/c mice were immunosuppressed with LPS. This was evaluated by cytometry, ELISA and hemagglutination. Results: KO mice were more sensitivity to LPS [mortality (LPS 10ug): WT 0/8; KO 8/8]. Also an elevated and sustained level of TNF-α up to 6 hours was observed [mean ± SEM TNF-α (pg/ml): WT= 731.8±9, KO= 3566±9; p