EXTRACELLULAR HISTONES EXERT A DIFFERENTIAL CYTOTOXIC AND ANTIANGIOGENIC EFFECT IN PROGENITOR AND MATURE ENDOTHELIAL CELLS

MENA, HA; CARESTIA, A; SCHATTNER, M; NEGROTTO, S

Toronto

Congreso; XXV Congress of the International Society on Thrombosis and Haemostasis (ISTH); 2015

EXTRACELLULAR HISTONES EXERT A DIFFERENTIAL CYTOTOXIC AND ANTIANGIOGENIC EFFECT INPROGENITOR AND MATURE ENDOTHELIAL CELLSHebe A. Mena* 1, Agostina Carestia1, Mirta Schattner1, Soledad Negrotto11Laboratory of Experimental Thrombosis, Institute Of Experimental Medicine, National Academy Of Medicine-Conicet,Buenos Aires, ArgentinaPreferred Presentation Method: Oral or PosterTravel Grant Application: Reach the WorldI confirm fulfilling the criteria for the Reach the World Award: YesCountry of origin: ArgentinaBackground: Histones are main components of neutrophil extracellular traps, which are highly augmented not only ininfection but also in sites of neovessel formation, like peritumoral and regeneration tissues. Extracelullar histone cytotoxiceffect on endothelial cells has been previously studied, although the mechanism involved are not fully understood.Aims: To further analyze histone cytotoxicity in endothelial cells and whether histones affect endothelial angiogenicfunctions.Methods: Endothelial cells from micro (HMEC1) and macrovascular (HUVEC) beds and late outgrowth endothelial colonyforming cells (ECFC) were treated with individual human recombinant histones. P