CONICET | Buscador de Institutos y Recursos Humanos

Neutrophil extracellular trap formation (NETs) in diabetes. Correlation with inflammatory markers.Agostina Carestia1, Gustavo Frechtel2, Gloria Cerrone2, Claudio Gonzalez3, Mirta Schattner1 and Patricia Casais1.1Laboratory of Experimental Thrombosis, Institute of Experimental Medicine-CONICET-National Academy of Medicine, Buenos Aires, Argentina.2Genetics and Molecular Biology, Department of Microbiology, Immunology and Biotechnology, School of Pharmacy and Biochemistry, University of Buenos Aires (UBA), Buenos Aires, Argentina.3Department of Pharmacology, School of Medicine, University of Buenos Aires (UBA), Buenos Aires, Argentina. The release of DNA-derived neutrophil extracellular traps (NETs) is a mechanism by which these cells kill bacteria and exert proinflammatory and prothrombotic activities. Diabetes is characterized by a chronic inflammatory state, endothelial dysfunction, an increased risk of infections rates and early cardiovascular disease. The aim of this study was to determine whether the ability of neutrophils to form DNA traps differs in diabetic patients from healthy controls, and the relationship between the presence of NETs with inflammatory biomarkers. Recently diagnosed diabetic patients (13) and healthy blood donors (13) were included. NET formation was studied by microscopy and using Syber Gold. Plasma levels of nucleosomes and von Willebrand factor (vWF) were measured by ELISA and P-selectin expression by cytometry. Microscopy studies showed that while neutrophils from healthy donors did not form NETs without stimulation, 9 out of 13 neutrophil samples from diabetic patients exhibited spontaneous NET formation. Also, analysis of free DNA revealed increased basal levels in patient?s samples (control:0.3±0.02 vs. patients:0.5±0.1ug/ml, p