DIVERGING BIOLOGICAL ROLES AMONG HUMAN MONOCYTE SUBSETS IN THE CONTEXT OF TB INFECTION

L.BALBOA; J. BARRIOS-BAYAN ; E. GONZALEZ-DOMINGUEZ ; C. LASTRUCCI; G. LUGO-VILLARINO ; D. MATAS-ESPINOZA ; P. SCHIERLOH ; D. KVIATCVOVSKY; O. NEYROLLES; I. MARIDONNEAU-PARINI ; C. SANCHEZ-TORRES ; R. HERNANDEZ-PANDO ; MC SASIAIN

Mar del Plata

Congreso; LXII Reunión Anual de la Sociedad Argentina de Inmunología; 2014

Sociedad Argentina de Inmunología (SAI)

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is one of the most devastating human diseases. In chronic infections such as TB, circulating monocytes (Mo) arrive to the infected site and give rise to macrophages and dendritic cells (DC). We demonstrated that human CD16pos Mo expand in TB patients, correlate with disease severity and are refractory to DC differentiation, suggesting diverging biological roles among Mo subsets. So, we investigated if human Mo subsets (CD16neg and CD16pos) are differentially recruited to lungs, and whether this event could affect the outcome of the infection in a humanized mice model. We found that CD16neg Mo were more prone to migrate in response to Mtb-derived gradients, while CD16pos Mo moved under steady state conditions, across naked inserts (P