Enhanced Chemotactic Response In Patients With Typical Hemolytic Uremic Syndrome

RAMOS MV; PANEK CA; RUGGIERI M; FERNANDEZ-BRANDO RJ; ABREY-RECALDE MJ; EXENI A,; LAZO MC; EXENI R; PALERMO MS

Innsbruck

Conferencia; 4th International Conference HUS-MPGN-TTP & related disorders, Hotel Grauer Bar,; 2013

HUS-MPGN-TTP european group

Monocytes (Mo) are central elements in the inflammatory response triggered during typical Hemolytic Uremic Syndrome (HUS). According their chemotactic receptor expression different subsets of Mo with distinct functional properties have been described in circulation. In healthy children (HC) around 80% of peripheral Mo are classical monocytes (CD14+CD16-) characterized as CCR2+CCR5-CCR1+ cells. Conversely the minor subpopulation of inflammatory monocytes (CD14+CD16+) expresses CCR5 and reduced levels of CCR2 and CCR1. Aim of the study: Considering that chemokine receptors such as CCR1, CCR2 and CCR5 participate in different renal diseases, the aim of this work was to analyze the involvement of chemokine receptors on Mo in HUS evolution. Patients: HUS patient in the acute period (n=12) and HC (n=15) were studied Method: The expression of CCR1, CCR2 or CCR5 in combination with CD16 was determined on Mo of whole blood by flow cytometry. In HUS patients, the level of expression was analyzed according days of anuria during the acute phase. Chemotactic activity of plasma samples from HUS or HC was also assayed. Results: HUS patients showed an increased expression of CCR1, CCR2 and CCR5 in the inflammatory subset of Mo, while in classical Mo only CCR5 was increased. The Mean Intensity Fluorescence (MIF) ±SD of CCR1/CCR2/CCR5 expression in Mo from HC vs HUS are shown: Classical Mo: HC= 17±3/34±4/5±1 vs HUS= 31±6/40±4/15±4*; inflammatory Mo: HC= 15±3/ 24± 4/13±1 vs HUS= 44± 8*/37±5*/30±5*), *p