Neutrophils interaction with human trophoblast cells and modulation by VIP.

CALO G; SABBIONE F; PAPARINI D; RAMHORST R; TREVANI AS; PEREZ LEIRÓS C

Los Cocos, Córdoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunología

The generation and maintenance of the maternal-placental interface involves a tight interaction between trophoblast cells (Tb) and different immune cell populations, mainly macrophages, NK and Treg. Neutrophils (neu) are detected in high numbers during secretory phase, decreasing in the first stages of pregnancy and increasing again towards the end to participate in the process of cervix maturation. Vasoactive Intestinal Peptide (VIP) is a vasodilator and immunomodulatory peptide, produced by Tb. Our aim is to study cellular and molecular mechanisms that determine the interaction of neu with macrophages and Tb in early gestation, focusing on the participation of VIP/VPAC system. Whole blood was obtained from healthy donors and neu were purified on a Ficoll-Triyosom gradient and Dextran sedimentation. We analyzed the effect of conditioned media (CM) of human Tb from 1st trimester (cell line Swan-71) on spontaneous apoptosis of neu, measured by fluorescence microscopy using ethidium bromide and acridine orange. We observed an increase in the % of apoptotic neu when they were incubated with CM of Tb(X±SE: MC:67±9,5%; basal:44±13,7%; P