Altered expression of chemokine receptors on monocytes during Hemolytic Uremic Syndrome.

RAMOS, MV; PANEK, CA; FERNANDEZ-BRANDO, RJ; EXENI, A; LAZO, MC; EXENI, R;; PALERMO, MS

Amsterdam, The Netherlands

Simposio; 8th International Symposium on Shiga toxin (Verocytotoxin) producing Escherichia coli Infections (); 2012

VTEC2012

Altered expression of chemokine receptors on monocytes during Hemolytic Uremic Syndrome.   Ramos, MV1; Panek, CA1; Fernandez-Brando, RJ1; Exeni, A2; Lazo, MC3; Exeni, R3; Palermo, MS1.   1Instituto de Medicina Experimental (IMEX)-CONICET, Academia Nacional Medicina, Buenos Aires, Argentina. 2Hospital Austral, Pilar, Buenos Aires, Argentina. 3Hospital Municipal del Niño, San Justo, Buenos Aires, Argentina.   Monocytes are central elements in the inflammatory response triggered during Hemolytic Uremic Syndrome (HUS). Different subsets of monocytes with distinct phenotypic and functional properties coexist in circulation. In healthy children (HC) around 80% of peripheral monocytes are CD14+CD16-, and are mainly characterized as CCR2+CCR5-CCR1+ cells (classical monocytes). Conversely the minor subpopulation, CD14+CD16+ cells (inflammatory monocytes), expresses CCR5 but reduced level of CCR2 and CCR1. Considering that chemokine receptors such as CCR1, CCR5 and CCR2 participate in different renal diseases, the aim of this work was to analyze whether the expression of these receptors is modulated in monocytes during HUS.     For this purpose, the expression of CCR1, CCR2 or CCR5 in combination with CD16 was determined on monocytes considered as CD14+ cells, on whole blood from HUS patients in the acute period and HC by flow cytometry.   HUS patients showed an expansion of inflammatory monocytes (%CD14+CD16+: HUS=14.8±3.5*, HC=3.5±0.5, n=8, *p