Assessment of Shiga toxin producing Escherichia coli O157:H7 pathogenicity in the presence of short chain fatty acids and lactate.

FERNANDO NICOLÁS SOSA; ROMINA JIMENA FERNÁNDEZ-BRANDO; MARÍA VICTORIA RAMOS; ALAN MAURO BERNAL; MARINA SANDRA PALERMO

Congreso; Reunión Anual de Sociedades de Biociencias (SAIC-SAI-AAFE-NANOMED.AR) 2021; 2021

Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 is a foodborne pathogen, which can lead to the life-threatening Hemolytic Uremic Syndrome (HUS). There is no treatment availablein order to reduce HUS outcome up to date. Short chain fatty acids (SCFAs), including acetate(A) and butyrate (B), are produced by the intestinal microbiota. Lactate (L) is the main metabolite of many fermented products. A, B and L can protect mucosa exposed to inflammatory insults. The aim of this work was to evaluate the effects of A, B and L on modulating STEC pathogenicity. We evaluated the inhibition of STEC growth after 3h culture, EspB production by SDS-PAGE and bacterial adherence to Caco-2 cells. We found that 50 mM of A and B, and 100 mM of the three compounds inhibited bacterial growth significantly compared to control: 0;1;10;50 and 100mM (median CFU(IQR) x109) A=2.2(1.8-2.7);2.3(2.0-2.6);2.2(1.8-2.4);1.4(1.2-2.0);1.1(0.5-2.0);B=2.1(1.9-2.3);1.9(1.8-2.2);1.6(1.5-2.0);1.3(1.0-1.4);0.5(0.4-1.0);L= 2.1(1.7-2.5);2.3(1.6-2.6);1.7(1.5-2.1);1.6(1.5-1.9);1.1(1.0-1.5); p