Tuberculosis Exacerbates HIV-1 Infection through IL-10/STAT3-Dependent Tunneling Nanotube Formation in Macrophages

SOURIANT, SHANTI; PINGRIS, KARINE; LASTRUCCI, CLAIRE; POINCLOUX, RENAUD; INWENTARZ, SANDRA; GONZÁLEZ-MONTANER, PABLO; VERGNE, ISABELLE; KURODA, MARCELO J.; MARIDONNEAU-PARINI, ISABELLE; SOURIANT, SHANTI; PINGRIS, KARINE; LASTRUCCI, CLAIRE; POINCLOUX, RENAUD; INWENTARZ, SANDRA; GONZÁLEZ-MONTANER, PABLO; VERGNE, ISABELLE; KURODA, MARCELO J.; MARIDONNEAU-PARINI, ISABELLE; BALBOA, LUCIANA; KVIATCOVSKY, DENISE; BAH, AICHA; RAYNAUD-MESSINA, BRIGITTE; POGGI, SUSANA; CORTI, MARCELO; ALLERS, CAROLINA; SASIAIN, MARIA DEL CARMEN; LUGO-VILLARINO, GEANNCARLO; BALBOA, LUCIANA; KVIATCOVSKY, DENISE; BAH, AICHA; RAYNAUD-MESSINA, BRIGITTE; POGGI, SUSANA; CORTI, MARCELO; ALLERS, CAROLINA; SASIAIN, MARIA DEL CARMEN; LUGO-VILLARINO, GEANNCARLO; DUPONT, MAEVA; COUGOULE, CÉLINE; GASSER, ROMAIN; AL SAATI, TALAL; MORAÑA, EDUARDO JOSE; LAGANE, BERNARD; KAUSHAL, DEEPAK; NEYROLLES, OLIVIER; VÉROLLET, CHRISTEL; DUPONT, MAEVA; COUGOULE, CÉLINE; GASSER, ROMAIN; AL SAATI, TALAL; MORAÑA, EDUARDO JOSE; LAGANE, BERNARD; KAUSHAL, DEEPAK; NEYROL

Cell Reports

Elsevier B.V.

Año: 2019 p. 3586 - 3599

2211-1247

The tuberculosis (TB) bacillus, Mycobacterium tuberculosis (Mtb), and HIV-1 act synergistically; however, the mechanisms by which Mtb exacerbates HIV-1 pathogenesis are not well known. Using in vitro and ex vivo cell culture systems, we show that human M(IL-10) anti-inflammatory macrophages, present in TB-associated microenvironment, produce high levels of HIV-1. In vivo, M(IL-10) macrophages are expanded in lungs of co-infected non-human primates, which correlates with disease severity. Furthermore, HIV-1/Mtb co-infected patients display an accumulation of M(IL-10) macrophage markers (soluble CD163 and MerTK). These M(IL-10) macrophages form direct cell-to-cell bridges, which we identified as tunneling nanotubes (TNTs) involved in viral transfer. TNT formation requires the IL-10/STAT3 signaling pathway, and targeted inhibition of TNTs substantially reduces the enhancement of HIV-1 cell-to-cell transfer and overproduction in M(IL-10) macrophages. Our study reveals that TNTs facilitate viral transfer and amplification, thereby promoting TNT formation as a mechanism to be explored in TB/AIDS potential therapeutics.