Crystal Violet Decolorization Assay for Rapid Detection of Multidrug-resistant Mycobacterium tuberculosis Isolates: A Multicenter Study

YILMAZ COBAN A; NADIA TAYYAB; IMPERIALE BELÉN; GUMRAL ALAKBAROVA; GOHAR ZAMAN; INGRID WAINMAYER; LUQQMAN SATTI; MORCILLO NORA; SIMBOLI NORBERTO

Inetrnational Journal of Mycobacteriology

Wolters Kluwer-Medknow Publications Wolters Kluwer India pvt.lta

Año: 2018 vol. 7 p. 310 - 314

2212-5531

Background: Effective control of tuberculosis is achieved by early diagnosis and drug susceptibility testing for initiation of appropriate treatment.The performance of crystal violet decolorization assay (CVDA) for susceptibility testing of Mycobacterium tuberculosis to isoniazid (INH) andrifampicin (RIF) was compared in a multicenter study. Methods: Seventy-two M. tuberculosis isolates were tested in two phases by CVDA.Results: In Phase I, the specifi city, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and agreement for INH were100%, respectively. Specifi city, sensitivity, PPV, NPV, and agreement for RIF were 98.2%, 100%, 94.1%, 100%, and 98.6%, respectively.In Phase II, specifi city, sensitivity, PPV, NPV, and agreement were 98%, 100%, 95.4%, 100%, and 98.6% for INH, respectively. Specifi city,sensitivity, PPV, NPV, and agreement for RIF were 96.3%, 88.2%, 88.2%, 96.3%, and 94.4%, respectively. Results in the study were obtainedon average 10.9 ± 3.1 days in Phase I and 9.8 ± 2.2 days in Phase II. Conclusions: CVDA can be performed for drug susceptibility testing indeveloped and developing countries. In addition, further studies with larger sample size are needed for evaluation of this method