IMEX 05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Anti-neuroblastoma properties of a recombinant sunflower lectin
Autor/es:
PINEDO, M.; DE OLIVEIRA CARVALHO, A.; SYLVEIRA, M.X.; DEL RIO, M.; GENOULA, M.; REGENTE, M.C.
Revista:
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Editorial:
MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI
Referencias:
Lugar: Basel; Año: 2017 vol. 18 p. 92 - 103
ISSN:
1422-0067
Resumen:
According to their sugar recognition specificity, plant lectins are proposed as bioactiveproteins with potential in cancer treatment and diagnosis. Helja is a mannose-specific jacalin-likelectin from sunflower which was shown to inhibit the growth of certain fungi. Here, we report itsrecombinant expression in a prokaryotic system and its activity in neurobalstoma cells. Helja codingsequence was fused to the pET-32 EK/LIC, the enterokinase/Ligation-independent cloning vectorand a 35 kDa protein was obtained in Escherichia coli representing Helja coupled to thioredoxin(Trx). The identity of this protein was verified using anti-Helja antibodies. This chimera, namedTrx-rHelja, was enriched in the soluble bacterial extracts and was purified using Ni+2-Sepharoseand D-mannose-agarose chromatography.
