Role of G protein coupled receptor kinase 2 in proliferation of pancreatic cells.

RIPOLL S; ECHEVERRIA E; BURGHI V; FERNANDEZ NC; DAVIO C

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

G protein-coupled receptor (GPCR) kinase 2 (GRK2) is known for its role in phosphorylation and desensitization of GPCRs. However, GRK2 has been shown to interact also with non-receptor targets and has been associated to cell migration and proliferation. Since GRK2 is upregulated in pancreatic ductal adenocarcinoma we aimed to evaluate the role of GRK2 in the regulation of proliferation of pancreatic BxPC3 cell line and to discriminate between phosphorylation dependent and independent mechanisms in this process. In MTS proliferation assays we found that transfection of BxPC3 with wild type GRK2 or kinase death mutant did not modify cell proliferation. However, transfection with a dominant negative mutant of the regulator of G protein signaling (RH) domain of GRK2 significantly decreased proliferation rate (100±15% mock vs 50±17% RHmut, P