Enhancement of glucocorticoid receptor transcriptional activity by CB1 cannabinoid receptor signaling. Its role on memory consolidation of aversive experiences.

GRANJA GALEANO, GINA; ZORRILLA ZUBILETE, MARIA; MONCZOR FEDERICO; DOMINGUEZ-RUBIO, ANA PAULA; FITZSIMONS CARLOS; ZAPPIA, CARLOS DANIEL; FRANCHI, ANA

Mar del Plata

Congreso; LXI Reunión Anual de la SAIC; 2016

SAIC

The cannabinoid receptor type 1 (CB1) and the glucocorticoid receptor (GR) are coexpressed in several brain areas involved in learning and memory, including the hippocampus, a brain area involved in spatial and episodic memory. Glucocorticoids mediate the behavioral consequences of stress, such as memory consolidation associated with emotionally arousing events. The downstream immediate-early gene Egr1 is a GR-responsive gene involved in memory consolidation. It has been suggested that CB1 signaling modulates GR-mediated transcriptional activity activity. Here, we studied the effect of CB1 signaling on GR-mediated transcriptional activity related to memory consolidation of arousing events. For in-vitro studies, we used the HT22 neuronal cell line, derived from murine hippocampus. In these cells, induction of Egr1 by the GR agonist Dexamethasone (Dex) was potentiated by the selective CB1 agonist meta-anandamide (300%, p