The construction of a limphocytic PGP activity data base with covariables.

ESCOBAR A., CORTADA C., CURRAS V., BRAMUGLIA G., CARBALLO M., NISELMAN A.V., RUBIO M. C.

Cordoba- Argentina

Congreso; XXXVIII reunión anual de la Asociación Argentina de Farmacolgía Experimental; 2006

Asociación Argentina de Farmacología Experimental

The construction of a limphocytic PGP activity data base with covariables. Escobar A., Cortada C., Curras V., Bramuglia G., Carballo M.,  Niselman  A.V., Rubio M. C. Departamentos de Farmacología y Matemáticas.  Citogenética Humana y Genética Toxicológica (CIGETOX), Fac. de Farmacia y Bioquímica, UBA, Junín 956 PB (1113) Buenos Aires, Argentina. Email:   vnisel@mybfyb.ffyb.uba.ar The aim of the work is to link the PGP  activity with the covariables measured in a sample of individuals. The sample size, in this initial stage was of 35 healthy individuals, men and women, between 20 and 60 years. The possible associations between the covariables and the PGP's fluorescence could allow us detecting regulatory factors. The analysis of  limphocytic PGP functionality is accomplished by measuring the  extrusion of Rodamina 123 followed by flow cytometry. This gives a value to which we call Fluorescente (FR). There were also measured three kind of covariables: biochemistries (hemogram, erythrocyte-sedimentation, hepatic enzymes, glycemia, total cholesterol, creatinine, triglycerides and bilirubin),  anthropometric (age, weight and sex) and clinics (diet, smoking habits, mate ingestion, coffee, tea and alcoholic drinks). The information was analyzed by means of stepwise multiple regression. The method express one variable (FR) like linear function of others in order to identify the important explanatory covariables (those with P< 0.05). We have found an association between fluorescence and cholesterol (P=0.008) and between fluorescence and age (P=0.04)  No other registered covariable was found related with PGP activity.