Analysis of telomere length in women with polycystic ovary syndrome

VELAZQUEZ MARIELA; MILLAN ANDREA; CERRONE GLORIA; VELAZQUEZ MARIELA

Congreso; SAIC-SAI-SAFIS 2018; 2018

Sociedad Argentina de Investigación Clínica (SAIC)

IntroductionPolycystic ovary syndrome (PCOS) is associated with metabolic disorders such as obesity, dyslipidemia, arterial hypertension and type 2 diabetes mellitus. Telomeric shortening is involved in the replicative capacity of a cell, so it becomes a marker of cell aging. Metabolic disorders, and hyperandrogenism that are frequently found in women with PCOS, can influence the length of telomeres (LT).Our objective was to determine the absolute LT (LTa) in women with PCOS in comparison with healthy controls and their association with metabolic variables and the presence of hyperandrogenism (HA).Materials and methodsWe analyzed 86 control women and 130 women with PCOS, between 16-46 years of age. Measurements of biochemical, clinical, anthropometric and hormonal variables were made. In particular, the total Testosterone value was considered higher than 0.9nG / mL as a parameter suggested by the different consensus to determine biochemical hyperandrogenism. The determination of the LTa was performed on genomic DNA extracted from peripheral blood leukocytes by the absolute quantitative method by real time quantitative PCR (Real Time PCR) by the SYBR Green method. The relationship between the kpb of telomeric sequences and the number of copies of the single copy gene RPLPO (radio T / S) was determined. Statistical analysis was carried out by one-way Anova and linear regression in SPSS version 20.0 with a significance level of 0.05.ResultsClinical characteristics of study participants: Compared to controls, patients with PCOS have a higher weight, body mass index (BMI), greater waist circumference (WC), higher levels of triglycerides (TG), and fasting plasma glucose (Glu). No differences were found in c- HDL, SBP and DBP levels among women with PCOS and controls.Association between LTa and age: An inverse relationship was observed between LTa and age (p = 0.004) in all the participants of our study.Association between LTL and the presence of PCOS: Women with PCOS presented a significantly higher LTa compared to controls (p = 0.001, adjusted for age: p = 0.005).Association of LTato metabolic variables: In women with PCOS, we found a significant association between higher LTa and lower BMI (p = 0.040), lower waist circumference (p = 0.004), lower TG levels (p = 0.049), lower PAD (p = 0.001) and higher c-HDL (p = 0.004).Association of LTato biochemical HA: Significant differences were found between women with SOP-HA compared with women SOP-NHA and Controls (p = 0.004), SOP-HA vs SOP-NHA (p = 0.192), SOP-HA vs Controls (p = 0.005) and SOP-NHA vs Controls (p = 0.083).ConclusionThe inverse relationship between LTa and age was verified in all the participants of our study. Although a significantly higher LTa was observed in women with PCOS compared to controls, this would be a consequence of the presence of different metabolic and hormonal components. A lower LTa was associated with the presence of different components of the metabolic syndrome, such as BMI, WC, TG, DBP and c-HDL in women with PCOS, while the presence of biochemical HA was associated with higher LTa. Our results contribute to knowledge about the role of LT in the pathophysiology of PCOS.