Maternal saturated fat rich diet programs impairment of hepatic lipid metabolism and detoxifying enzymes expression in offspring

VERÓNICA WHITE; MARÍA BELÉN MAZZUCCO; EVANGELINA CAPOBIANCO; ALICIA JAWERBAUM

Mar del Plata

Congreso; VI Latin American Symposium on Maternal Fetal Interaction and Placenta (SLIMP); 2015

Diets enriched in saturated fat alters metabolism. During development (gestation and lactation) an overload in saturated fat can disturb maternal and fetal metabolism determining metabolic anomalies in the offspring. The liver is an organ involved in detoxification of drugs and oxidized toxic moieties. Multidrug resistant proteins (MRP) are transporters involved in the clearance process and related to antioxidant effects. Objective: To investigate the effects of a saturated fat overload on maternal diet on lipid accumulation, oxidative stress induction and detoxifying enzymes expression in the liver from 21- and 130-day-old offspring. Methods: Female Wistar rats were fed with a standard diet (6% fat) or with a saturated fat diet (30% fat) as from 6 weeks of age. After 8 weeks, they were mated with control males. Rats were allowed to deliver and to breastfeed their pups. Male and female offspring was euthanized at 21 and 130 days old. Livers were explanted, weighed and preserved for the analysis of lipid accumulation (TLC), lipid peroxidation (TBARS), nitric oxide production (nitrates-nitrites evaluation, Griess assay) and MRP2 and MRP4 expression (PCR). Results: We found triglycerides overaccumulation in livers from 21- (50%, p 0.05) and 130- (54%, p 0.05) day-old offspring from saturated fat-fed rats when compared to controls. Livers from 21-day-old offspring from saturated fat-fed rats showed an increase in nitrates/nitrites concentrations (200%, p 0.001), TBARS (200%, p 0.01) and a decrease in MRP2 expression (25%, p 0.05) when compared to controls. Similar results were evident in 130-day-old offspring from saturated fat-fed and control rats. Conclusions: A maternal saturated fat-enriched diet induced anomalies in liver metabolism that persisted in the offspring. Overaccumulation of lipids, NO overproduction, oxidative stress and diminished MRPs expression could lead to hepatic dysfunction and impair proper detoxification of the organism