CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EFFECT OF DEHYDROEPIANDROSTERONE AND METFORMIN IN CORPUS LUTEUM REGRESSION.
Autor/es:
ALICIA BEATRIZ MOTTA; VALERIA SANDER; GRACIELA FACORRO; LIDIA PIEHL; EMILIO RUBIN DE CELIS
Lugar:
Hotel Marriott, San Franciasco, California, USA
Reunión:
Congreso; 6 th Annual Meeting - Androgen Excess & PCOS Society San Francisco California, noviembre 2008; 2008
Institución organizadora:
Androgen Excess & PCOS Society
Resumen:
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EFFECT OF DEHYDROEPIANDROSTERONE AND METFORMIN IN CORPUS LUTEUM
REGRESSION.
Motta AB(1), Sander VA(1),
Facorro G(2), Piehl L(2), Rubin de Celis E(2)
(1)Laboratorio de Fisio-patología
Ovárica, CEFYBO-UBA,CONICET-Buenos Aires, Argentina; and (2)Dept. of Física,
Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, Argentina
Hyperandrogenism alters ovarian function
leading to an abnormal length of estrus cycles, anovulation and high rates of
recurrent miscarriages.
The aim of the present study was to
investigate the action of androgens on corpus luteum (CL) regression and the
mechanism by which N, N- dimethylbiguanide metformin restores cyclicity.
The injection of dehydroepiandrosterone
(DHEA; 60 mg/Kg body weight) to BALB/c mice 24 and 48 hours before luteolysis
increased serum progesterone and estradiol levels and decreased ovarian
oxidative stress (since DHEA decreased ovarian lipid peroxidation and increased
superoxide dismutase and catalase activities). Therefore, the treatment with
DHEA diminished the ovarian production of the luteolytic prostaglandin (PG) F2
alpha and increased both the ovarian levels of the luteotrophic PGE and the ovarian
nitrite concentration. We also found that the effects of DHEA on NO production
involved the regulation of the corresponding main enzymes of this pathway.
Metformin prevented the action of DHEA on
ovarian oxidative stress, progesterone levels, PGs production and NO levels also
involving the corresponding enzymes.
We conclude that hyperandrogenism is able
to delay the luteolytic process. This observation could explain, in part, the
abnormal length of the sexual cycle of women with PCOS. We also reported novel
mechanisms by which metformin is able to act as an anti-inflammatory and
antioxidant drug during the processes of hyperandrogenism. (supported in part
by ANPCyT grants PICTR 32529 and PICT 949).