DIVERGENT ROLES OF GALECTIN-1 AND GALECTIN-3 IN THE CONTROL OF OLIGODENDROCYTE

PASQUINI , J.M., MILLET, V., GIANNONE, J.P., BIANCO, G., SOTO,E.F., RABINOVICH, G.A., PASQUINI, L.A.

Charleston, South Carolina, EEUU

Congreso; ASN 40th annual meeting; 2009

American Society of Neurochemistry

Galectins are a family of lectins phylogenetically conserved and defined as a shred consensus sequence and a carbohydrate recognition domain sponsible for the binding to b-galactosidase. Until present, it has been described 10 different subfamilies localized in an extensive number of species and tissues which have been classified according to their biochemical structure.Their extensive distribution in nature and theirconserved aminoacidic sequences through evolution suggest that these proteins could perform essential physiological roles. In the present investigation we focus basically on the biological effects of Gal-1 and Gal-3 on oligodendroglial cell (OLGc) survival and differentiation. Our results in OLGc primary cultures show that there is an increase in the expression of Gal-3 during OLGc maturation and that treatment of the cultures with recombinant Gal-3 produces a significant increase in MBP1cells as well as a decrease in the number of A2B51cells. Contrarywise recombinant Gal-1 added to OLGc cultures produce a diminished expression of the different MBP isoforms. Finally analysis of the levels of the different MBP mRNAs and MBP isoforms in Gal-1 knock out mice during the myelination period showed no changes in these parameters compared with those observed in wild type control mice.b-galactosidase. Until present, it has been described 10 different subfamilies localized in an extensive number of species and tissues which have been classified according to their biochemical structure.Their extensive distribution in nature and theirconserved aminoacidic sequences through evolution suggest that these proteins could perform essential physiological roles. In the present investigation we focus basically on the biological effects of Gal-1 and Gal-3 on oligodendroglial cell (OLGc) survival and differentiation. Our results in OLGc primary cultures show that there is an increase in the expression of Gal-3 during OLGc maturation and that treatment of the cultures with recombinant Gal-3 produces a significant increase in MBP1cells as well as a decrease in the number of A2B51cells. Contrarywise recombinant Gal-1 added to OLGc cultures produce a diminished expression of the different MBP isoforms. Finally analysis of the levels of the different MBP mRNAs and MBP isoforms in Gal-1 knock out mice during the myelination period showed no changes in these parameters compared with those observed in wild type control mice.