HYPOXIA-ISCHEMIA-MEDIATED OLIGODENDROGLIAL CELL DEATH AND ITS RECOVERY BY APOTRANSFERRIN

GUARDIA CLAUSI, M., PASQUINI, L.A., SOTO, E.F., PASQUINI, J.M.

Charleston, Carolina del Sur, EEUU

Congreso; 40st Annual Meeting of AMERICAN SOCIETY FOR NEUROCHEMISTRY; 2009

AMERICAN SOCIETY FOR NEUROCHEMISTRY

We have previously demonstrated that apotransferrin (aTf) intracranially injected (ICI) in rats at 3 days of age produces an accelerated oligodendroglial cell (OLGc) maturation (Marta et al., 2000). To investigate if aTf provides neuroprotection to oligodendrocyte precursor cells (OPCs) following cerebral hypoxia-ischemia (H/I), we used a neonatal rat model of white matter damage previously developed by Vanucci et al., 2005. Hypoxia-ischemia produced a severe white matter damage, evaluated by Perl stain, as soon as 48 hours after H/I. Iron staining in the corpus callosum (CC) was maximum four days after H/I. and an increased ferritin expression paralleled iron accumulation. A decrease in O41cells and mature OLGc markers such as carbonic anhydrase II (CAII) and RIP and an increase in immature OLGc markers such as NG2 as well as an increase in astrocytes (evaluated by GFAP immunoreactivity) was also observed two weeks after H/I. Three days after H/I an increased number of nestin positive cells appeared in the SVZ and an increased number of PDGFRa in the SVZ was also observed after the injection of aTf. As was alreadydescribed, apotransferrin is probably producing an accelerated maturation of an increased number of oligodendrocyte precursor cells (OPC) induced by H/I. Other possible mechanisms involved will be also discussed. A decrease in O41cells and mature OLGc markers such as carbonic anhydrase II (CAII) and RIP and an increase in immature OLGc markers such as NG2 as well as an increase in astrocytes (evaluated by GFAP immunoreactivity) was also observed two weeks after H/I. Three days after H/I an increased number of nestin positive cells appeared in the SVZ and an increased number of PDGFRa in the SVZ was also observed after the injection of aTf. As was alreadydescribed, apotransferrin is probably producing an accelerated maturation of an increased number of oligodendrocyte precursor cells OPC) induced by H/I. Other possible mechanisms involved will be also discussed.