The application of Capillary Electrophoresis to evaluate the adsorption of organic compounds and their degradation products employing Fe3O4 Magnetic Nanoparticles coated with Humic Acids

PAULA DABAS; ROMINA CARBALLO; FABIOLA KAMECKI GONZALEZ

Santiago

Simposio; 22nd Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2016

Dyes are commonplace and an important class of compounds that have found a widespread use within society. Malaquite Green (MG) is a triphenylmethane dye that may be used mainly as a fungicide and parasiticide in aquaculture. But also, it has toxic effects that increase with concentration, exposure time and temperature. Due to its toxicity, the Food and Drug Administration nominated MG as a priority chemical for carcinogenicity testing in 1993 [1]. Nanoparticles have special physical and chemical properties, which determine a particular behavior in environmental systems and organisms. Fe3O4 magnetic nanoparticles (MNPs) have attracted significant interest in many fields due to their advantages such as facile recovery and recycling. The macromolecular humic acids (HA) can form complexes with iron and iron oxide through carboxylate, phenolic, and carbonyl functional groups in HA molecule [2]. The aim of this work is to combine the properties of both AH and MNPs for contamination removal. The Fe3O4 magnetic nanoparticles coated with humic acid and bare MNPs was prepared by coprecipitation, both were characterized by IR and Zeta Potential. The adsorption experiments were carried out with MNPs stabilized or not with HA, aqueous solution of MG, in the presence of Cu2+ and H2O2. The degradation process was evaluated by capillary electrophoresis. This analysis was performed in a Beckman P/ACE MDQ. An untreated fused-silica capillary of 75 μm i.d. x 375 μm o.d. was used and the effective length was 50 cm to the detector. The mobile phase consisted of 50 mM ammonium acetate buffer (pH 9.0), acetonitrile 15% (v/v). Aliquots were taken at different times and introduced into CE by 5 s at 3.5 kPa and the separation voltage was 20 kV. The CE system temperature was held at 20 °C and UV detection at 580 nm was performed. The Fe3O4 magnetic nanoparticles coated with humic acid were able to remove 95.5% of MG in water, while bare MNPs only can remove 27.1% in the same time. Thus, they showed to be more efficient in removing MG than the bare MNPs. References: 1. Srivastava S, Sinha R, Roy D. Aquatic Toxicol. 2004, 66, 319-329. 2. Liu JF, Zhao ZS, Jiang GB. Environ. Sci. Technol. 2008, 42, 6949-6954.