Subventricular zone- derived progenitor cells and oligodendroglial differentiation in the adult rat brain

SILVESTROFF, L., FRANCO, P., BARTUCCI, S., SOTO, E. AND PASQUINI, J.

Buzios, Rio de Janeiro, Brasil

Congreso; I IBRO/LARC Congress of neurosciences of Latin America, the Caribean and Iberian Peninsula; 2008

IBRO/LARC

Adult rat SVZ tissue reacts to demyelinating stimuli observed by an increase in cell proliferation. We used Nestin antigen as an undifferentiated NPC marker and showed these cells increase in number in the SVZ of demyelinated animals. Oligodendrocytes derived from CPZ-exposed SVZ tissue develop phenotypes that express higher MBP levels in vitro, compared to controls, indicating that either CPZ or the demyelinating environment have an impact on undifferentiated NPC. SVZ tissue cultured in vitro continues expressing Nestin and GFAP under proliferating conditions which confirms the presence of NPC. Explant-derived Nestin+ cells migrate extensively in a radial manner away from the center of the explant and cell morphology changes as this takes place. Once mitogens are removed from culture media cells adopt astrocyte and oligodendroglial morphologies that were later identified as such by ICC analysis (data not shown). Neurosphere-derived cells can differentiate into MBP expressing oligodendrocytes. NPC exposure to Tf or PDGF, either during proliferating or differentiating conditions, show oligodendrocytes express higher MBP levels than untreated cell cultures. Our main aim is to set the bases from which we plan to study Tf and PDGF, and their possible interactions, at a molecular level that could explain their effects during cell commitment and oligodendroglial maturation.