Peripheral remyelination: evidence of a temporal window for Bone Marrow Mononuclear Cell transplantation

USACH, VANINA; PIÑERO, GONZALO; SOTO, PAULA; ROZENSZAJN, MIJAEL; ERIC GAMBERG; CUETO ALICIA; SETTON - AVRUJ, PATRICIA

Mar del Plata, Argentina

Congreso; XXX Congreso Anual Sociedad Argentina de Neurociencias (SAN); 2015

We have previously described the reorganization of major myelin proteins (MBP and P0), as well as the axonal protein PGP 9.5 in the ipsilateral nerve during the demyelination period and the migration of bone marrow mononuclear cells (BMMC) exclusively to the injured nerve in a reversible model of Wallerian degeneration (WD). Once BMMC arrived at the ipsilateral nerve, some of them colocalized with Schwann cell and nerve fiber markers, and accelerate the regeneration process.The aim of the present work was to evaluate if there is a temporal window during which BMMC are able to migrate to the injured nerve and if this migration is also accompanied by a beneficial effect. To that end, adult Wistar rats were submitted to sciatic nerve crush and at different survival times post crush, they were transplanted with BMMC. 5 days after transplantation, animals were sacrificed and immunohistochemistry, Western Blot and functional studies were performed.Results shown that BMMC can migrate to the ipsilateral nerve up to 28 days post crush, but the peak of migration occurred when BMMC were transplanted 7 days post crush, which correspond to the peak of demyelination. Although these treatment seems to produce some beneficial effects, it less that the one observed when transplantation occurred immediately after crush.More studies should be done to determine if the existence of a temporal window for BMMC transplantation, leads to a faster regeneration than the one observed without treatment.