MOLECULAR MARKERS IN CHILDREN PAROTID SALIVA. A PROTEOMIC APPROACH.

1FERRARY, TERESITA; 2CORTEZ, LEONARDO; 2VINZÓN, SABRINA, 2BISCOGLIO DE JIMÉNEZ BONINO, MIRTHA

Valencia, España

Congreso; Joint Congress SEProt-EuPA on Proteomics & Pathology -from both sides of the; 2007

SEProt-EuPA

The “normal” protein pattern of physiological fluids can be used as reference for differential diagnosis of pathologies. Saliva proteome is composed by a number of proteins involved in the homeostasis of the oral cavity. The main objectives of this work are: a) to describe the protein profile of parotid saliva of healthy children; b) to identify molecular markers for juvenile rheumatoid arthritis (JRA). Ten samples of parotid saliva of healthy children and ten samples of children with juvenile rheumatoid arthritis were collected with a Lashley cup following stimulation with a 2 % citric acid solution. After molecular exclusion chromatography, each fraction was submitted to HPLC-ESI-MS. The fraction containing 10-23 kDa molecular species was analyzed by RP-HPLC. A further MS analysis as well as 2D SDS-PAGE allowed detection of a group of proteins that may be considered molecular markers of the “normal” children parotid saliva. Those samples of saliva from children with JRA were submitted to the same procedure; a 18.46 kDa protein was present in most of samples studied thus indicating that the method could be a new tool for the diagnosis of this disease.