BRADYKININ (BK) INDUCES AN EPITHELIALMESENCHYMAL TRANSITION IN NEPHROGENIC URETERICBUD(UB)CELLS

GUAYTIMA, EV; BRANDAN, YR; FAVALE, NO; STERIN SPEZIALE, NB; MARQUEZ, MG

Mendoza

Congreso; XLVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); 2012

SAIB

We studied the effect of BK on renal papillary collecting duct/UB cells of 7 days-old rats. In mammals, nephrogenesis is completed posnatally. Cultured UB cells forms large colonies with a well spread morphology and lamellipodia in the peripheral cells showing the phenotype of a migratory sheet of epithelial cells. UB cells interact with each other and with the substratum throughadherens junctions and focal adhesions (FA) immunostained with vinculin. BK treatment resulted in cell scattering and FAdisipation, accompanied by changes in cellular morphology from a spread to a more rounded shape. We also observed irregular cells with lamellipodia and filopodia making contacts with neighbouring cells. Pre-treatmente of cells with a selective BK-B2 receptor antagonist, Hoe 140, avoid cell scattering and FA dissipation. We interpret that in the absence of BK, UB cells form colonies and migrate collectively, and this behavior could explain the collectiveadvance of the UB from papilla to renal cortex which take place in nephrogenesis. Taking into account that BK-B2 mRNA levels are higher in newborn kidney, we propose that in kidney development BKinduces epithelial- mesenchymal transition, which causes to the cells loosen their junctional interactions and become migratory mesenchymal-like cells, which finally could undergoesmesenchymal–epithelial conversion to gives rise kidney tubules