IQUIFIB 02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role Of Vinculin In The Process Of Collective Cell Migration Of The Nephrogenic Ureteric Bud (Ub)
Autor/es:
MARQUEZ MG; GUAYTIMA E; BRANDAN YR; FAVALE NO; PESCIO L; STERIN-SPEZIALE NB
Lugar:
Puerto Madryn, Chubut, Argentina
Reunión:
Congreso; XLVI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; 2010
Institución organizadora:
SAIB
Resumen:
In mammals, nephrogenesis is completed
posnatally. In this work, we studied the role of vinculin in the process of
collective migration of UB cells. We made primary cultures of renal papillary
collecting duct/UB cells of 7 days-old rats, and studied the expression of
vinculin and actin cytoskeleton by immunofluorescence. We observed isolated
cells making contact by filopodia and long membrane prolongations, and colonies
of cells with migratory phenotype. Some border cells contained greater amount
of vinculin than the rest of cells, and marginal semicircular bundles of actin,
recruited by vinculin located in the plasma membrane. A selective Rho-kinase
inhibitor abrogated the accumulation of vinculin in border cells. These results
indicate that UB cells display a great capacity to form colonies and to migrate
collectively. We interpret that vinculin accumulation, and the recruitment of
actin filaments, only occurs in those cells whose function is the one to
indicate the direction of the collective migration of the UB cells, which is a
Rho - dependent mechanism. This behavior could explain the advance of the UB
from papilla to renal cortex where takes place the mutual induction between the
metanephric mesenchyme (MM) and UB cells, and as a result MM undergoes
mesenchymalepitelial conversion to gives rise to the nephron, and the UB gives
rises to the renal collecting duct system.
