IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Modulation of endothelial cell migration and angiogenesis: A novel
Autor/es:
VÍCTOR MANUEL CÁRDENAS DELGADO; LUCAS COLOMBO; MARÍA FERNANDA TRONCOSO; LORENA NUGNES; MARISA FERNÁNDEZ; EMILIO MALCHIODI; ISABEL FRAHM; DIEGO CROCI; DANIEL COMPAGNO; GABRIEL ADRIÁN RABINOVICH; CARLOTA WOLFENSTEIN-TODEL; MARÍA TERESA ELOLA
Revista:
FASEB JOURNAL
Editorial:
FEDERATION AMER SOC EXP BIOL
Referencias:
Lugar: Bethesda, Maryland; Año: 2010
ISSN:
0892-6638
Resumen:
Abstract
Angiogenesis, the growth of new capillaries from pre-existing blood vessels, is a
complex process involving endothelial cell (EC) activation, disruption of vascular
basement membranes, migration and proliferation of ECs. Glycan-mediated recognition
has been proposed to play an instrumental role in mediating cell-cell and cell-matrix
interactions. Galectins (Gal), a family of glycan-binding proteins with affinity for â-
galactosides and a conserved sequence motif, can decipher glycan-containing
information and mediate cell-cell communication. Galectin-8 (Gal-8), a member of this
family, is a bivalent tandem-repeat-type galectin, which possesses two CRDs
connected by a linker peptide. Here, we show that Gal-8 is endowed with proangiogeneic
properties. Functional assays revealed a critical role for this lectin in the
regulation of capillary-tube formation and EC migration. Moreover, matrigel, either
supplemented with Gal-8 or vascular endothelial growth factor (VEGF), injected in
mice resulted in induction of in vivo angiogenesis. Remarkably, Gal-8 was expressed
both in the cytoplasm and nucleus in ECs of normal and tumor vessels. Furthermore,
CD166 (ALCAM, activated leukocyte cell adhesion molecule) was identified as a
specific Gal-8-binding partner in normal vascular ECs. Collectively, these data provide
the first evidence demonstrating an essential role for Gal-8 in the regulation of
angiogenesis with critical implications in tumor biology.