IQUIFIB 02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
artículos
Título:
Membrane Lipid Composition Plays a Central Role in the Maintenance of Epithelial Cell Adhesion
Autor/es:
MARÍA G MÁRQUEZ; FRANCISCO LEOCATA NIETO; MARÍA C. FERNÁNDEZ TOME; NICOLÁS O. FAVALE; NORMA B. STERIN-SPEZIALE
Revista:
LIPIDS
Editorial:
Springer
Referencias:
Año: 2008 vol. 43 p. 343 - 352
ISSN:
0024-4201
Resumen:
Focal contacts (FC) are membrane-associatedmulti-protein complexes that mediate cell-extracellularmatrix (ECM) adhesion. FC complexes are inserted indetergent-resistant membrane microdomains enriched inphosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2);however, the influence of membrane lipid composition inthe preservation of FC structures has not been extensivelyaddressed. In the present work, we studied the contributionof membrane lipids to the preservation of renal epithelialcell adhesion structures. We biochemically characterizedthe lipid composition of membrane-containing FC complexes.By using cholesterol and PtdIns(4,5)P2 affectingagents, we demonstrated that such agents did not affect anyparticular type of lipid but induced the formation of newFC-containing domains of completely different lipid
