MACNBR   00242
MUSEO ARGENTINO DE CIENCIAS NATURALES "BERNARDINO RIVADAVIA"
Unidad Ejecutora - UE
artículos
Título:
Nuclear Factor NF-kB controls expression of the immunoregulatory glycan-binding protein galectin-1
Autor/es:
TOSCANO MA; CAMPAGNA L; MOLINERO L; CERLIANI JP; CROCI DO; ILARREGUI JM; FUERTES MB; NOJEK IM; FEDEDA JP; ZWIRNER NW; COSTAS MA; RABINOVICH GA
Revista:
MOLECULAR IMMUNOLOGY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2011 vol. 48 p. 1940 - 1940
ISSN:
0161-5890
Resumen:
The inflammatory response is a self-limiting process which involves the sequential activation of signaling pathways leading to the production of both pro- and anti-inflammatory mediators. Galectin-1 (Gal-1), an endogenous lectin found in peripheral lymphoid organs and inflammatory sites, elicits a broad spectrum of biological functions predominantly by acting as a potent anti-inflammatory factor and as a suppressive agent for T-cell responses. However, the molecular pathways underlying Gal-1 expression and function remain poorly understood. Here, we identified a regulatory loop linking galectin-1 expression and function to NF-£eB activation. NF-£eB-activating stimuli differentially increased Gal-1 expression in T cell subsets, an effect which could be selectively prevented by inhibitors of NF-£eB. Accordingly, transient transfection of the p65 subunit of NF-£eB was sufficient to induce high Gal-1 expression. Using in silico studies and chromatin immunoprecipitation analysis we have identified a functional NF-£eB binding site within the first intron of the LGALS1 gene. In addition, our results show that exogenous Gal-1 can attenuate NF-£eB activation, as shown by inhibition of I£eB-£ degradation induced by pro-inflammatory stimuli, higher cytoplasmic retention of p65, lower NF-£eB DNA binding activity and impaired transcriptional activation of target genes. The present study reveals a novel regulatory loop by which NF-£eB induces expression of Gal-1, which in turn may lead to negative control of NF-£eB signaling.