MACNBR   00242
MUSEO ARGENTINO DE CIENCIAS NATURALES "BERNARDINO RIVADAVIA"
Unidad Ejecutora - UE
artículos
Título:
DNA barcodes provide new evidence of a recent radiation in the genus Sporophila (Aves: Passeriformes)
Autor/es:
CAMPAGNA L.; LIJTMAER, D. A.; KERR, K. C. R.; BARREIRA, A.; HEBERT, P. D. N.; LOUGHEED, S. C.; TUBARO, P. L.
Revista:
MOLECULAR ECOLOGY RESOURCES
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 10 p. 449 - 458
ISSN:
1755-098X
Resumen:
The capuchinos are a group of birds in the genus Sporophila that has apparently radiated
recently, as evidenced by their lack of mitochondrial genetic diversity. We obtained cytochromeSporophila that has apparently radiated
recently, as evidenced by their lack of mitochondrial genetic diversity. We obtained cytochrome
c oxidase I (COI) sequences (or DNA barcodes) for the 11 species of the group and
various outgroups.We compared the patterns of COI variability of the capuchinos with those
of the largest barcode data set from neotropical birds currently available (500 species representing
51% of avian richness in Argentina), and subjected COI sequences to neighbourjoining,
maximum parsimony and Bayesian phylogenetic analyses as well as statistical parsimony
network analysis. A clade within the capuchinos, the southern capuchinos, showed
higher intraspecific and lower interspecific divergence than the remaining Argentine species.
As most of the southern capuchinos shared COI haplotypes and pairwise distances within
species were in many cases higher than distances between them, the phylogenetic affinities
within the group remained unresolved. The observed genetic pattern is consistent with both
incomplete lineage sorting and gene flow between species. The southern capuchinos constitute
the only large group of species among the neotropical birds barcoded so far that are
inseparable when using DNA barcodes, and one of few multispecies avian groups known to
lack reciprocal monophyly. Extending the analysis to rapidly evolving nuclear and mitochondrial
markers will be crucial to understanding this radiation. Apart from giving insights into
the evolution of the capuchinos, this study shows how DNA barcoding can rapidly flag species
or groups of species worthy of deeper study.oxidase I (COI) sequences (or DNA barcodes) for the 11 species of the group and
various outgroups.We compared the patterns of COI variability of the capuchinos with those
of the largest barcode data set from neotropical birds currently available (500 species representing
51% of avian richness in Argentina), and subjected COI sequences to neighbourjoining,
maximum parsimony and Bayesian phylogenetic analyses as well as statistical parsimony
network analysis. A clade within the capuchinos, the southern capuchinos, showed
higher intraspecific and lower interspecific divergence than the remaining Argentine species.
As most of the southern capuchinos shared COI haplotypes and pairwise distances within
species were in many cases higher than distances between them, the phylogenetic affinities
within the group remained unresolved. The observed genetic pattern is consistent with both
incomplete lineage sorting and gene flow between species. The southern capuchinos constitute
the only large group of species among the neotropical birds barcoded so far that are
inseparable when using DNA barcodes, and one of few multispecies avian groups known to
lack reciprocal monophyly. Extending the analysis to rapidly evolving nuclear and mitochondrial
markers will be crucial to understanding this radiation. Apart from giving insights into
the evolution of the capuchinos, this study shows how DNA barcoding can rapidly flag species
or groups of species worthy of deeper study.