INVESTIGADORES
CHIRDO Fernando Gabriel
congresos y reuniones científicas
Título:
Analysis of Draining Leukocytes from the Abdominal Fluid for monitoring Immune Events after Intestinal Transplant
Autor/es:
MEIER D; CAGNOLA HH; RAMISCH D; RUMBO C; CHIRDO F; DOCENA G; GONDOLESI G; RUMBO M
Lugar:
Bologna
Reunión:
Congreso; XIth International Small Bowel Transplant Symposium; 2009
Institución organizadora:
XIth International Small Bowel Transplant Symposium
Resumen:
Introduction:
During intestinal transplant (ITx) operation, intestinal lymphatics are not
reconstituted. Our aim was to evaluate whether leukocytes migrating from the
transplanted intestine could be recovered from the abdominal draining fluid and
to determine the potential applications of the assessment of draining cellular
populations to basic and clinical studies. Methods: Six consecutive ITx
patients were included; the cell composition of the abdominal draining fluid
was analyzed the first 14 post-ITx days by flow cytometry. Cell sorting and
molecular fingerprinting by short tandem repeat amplification was performed.
The correlation between analyzed parameters and clinical evolution was
evaluated. Results: In spite of marked lymphopenia in peripheral blood,
all patients analyzed showed a variable proportion of lymphocytes in abdominal
draining fluid, that in all cases was of above the 50% of total cells present
in this fluid. The main populations in the draining fluid were CD3+CD4+CD8-,
CD3+CD8+CD4- and CD3-HLA-DR+CD19+
lymphocytes. Cellular pattern varies along the post-ITx period in
non-complicated recipients from a mixed leukocyte pattern to an exclusively
lymphocytic pattern (Fig1, a-c). We could associate changes in draining cell
patterns to early events such as rejection or infections. Graft derived
lymphocytes were recognized by genetic fingerprinting of CD8+ sorted
T cells. At days 1-2, donor T cells were detected in the draining fluid (50% of
total CD8+ cells) and were mostly replaced by day 11 after ITx (<2%),
confirming that cells migrating from the graft can be recovered in the draining
fluid.
Conclusions:
This study demonstrates that the cell analysis of the draining fluid from ITx
recipients may provide a useful approach for monitoring changes in graft
immunobiology during the first 3 weeks post-transplant. This type of analysis
might be serve to improve clinical management.