SOY PROTECTS AGAINST CADMIUM INTOXICATION IN LUNGS. EFFECTS ON ANTIOXIDANT RESPONSE AND HEAT SHOCK PROTEINS (HSP) EXPRESSION

MARTIN MOLINERO GD; PENACCHIO G; BOLDRINI GG; FANELLI M; GOMEZ NN; PEREZ CHACA MV; CORDOBA ME; ALVAREZ SM

Virtual

Congreso; Reunión Anual Sociedades de Biociencias; 2020

Sociedad de Investigación Clinica

Cadmium is a toxic metal and a widespread contaminant. The mechanisms ofits toxicity are not yet fully understood, but it has been shown that cadmiumincreases lipid peroxidation and oxidative stress in many organs. On the otherhand, it has been shown that soy consumption has many benefits for human health.Heat shock proteins are a superfamily of proteins that have an important rolein folding/degradation of proteins. Their expression can be used as a sensitivebiomarker when cells are under stress conditions. Antioxidant response includesenzymatic and non-enzymatic systems. These systems exert a protective effectagainst free radicals and oxidative damage. We decided to study the expressionof Hsp and the antioxidant response in rat lungs under cadmium intoxication,analyzing the possible protective effects of a soy based diet. 4 lots of adultWistar rats were used: 2 lots received casein and 2 lots, soy as proteinsource. Within each group, 1 lot received regular tap water and the other 15ppmof Cd (as CdCl2) in drinking water for 60 days. Lungs were removed,fixed and paraffin embedded. Immunohistochemistry was realized using Hsp27 andHsp70 antibodies. Total RNA was obtained using QuickZOL reagent, retrotranscribedand used for PCR amplification of GPx and SOD-2. CAT activity was measured inlungs homogenate. Results showed that Hsp27 expression increased in both Cdintoxicated groups (p