SOY BASED DIET MODIFIES CADMIUM-INDUCED LIPID METABOLISM ALTERATIONS IN RAT CEREBELLUM

MICHEL MC; MARTIN MOLINERO GD; ALVAREZ SM; BOLDRINI GG

Mar del Plata

Congreso; Reunion Conjunta de Sociedades de Biociencias; 2019

SAIC

Cadmium is a toxic agent that is also an environmental contaminant. We studied its effects and the protective role of a vegetarian versus animal protein diet in cerebellum. The lipid profile, lipoperoxidation and the expression of lipid metabolism related enzymes were analized. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (So) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. The animals weight was weekly measured. Cerebellum homogenates were used for TBA assay, and the levels of lipid peroxidation products - mainly malondialdehyde (MDA) - were determined spectrophotometrically as TBARSs. Lipids were extracted and total cholesterol (TC), triglycerides (TG) and phospholipids (PL) were determined. Total RNA was isolated with Trizol and cDNA was obtained. Cytidylyltransferase (CT), hidroxymethylglutaryl CoA reductase (HMGCoAR), fatty acid synthetase (FAS) and acetyl CoA catboxylase (ACC) were determined by PCR. S28 was used as an internal control. Cadmium concentration was determined with ICP-MS. Animals weight showed no differences among the groups. Cadmium concentrations showed an increase in CasCd vs. CasCo (0,001) with no differences among Soy groups. Total lipids content showed a trend to decrease in Soy groups compared to Casein groups, being only significant in SoCd vs. CasCd (p