IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
-CADMIUM CHRONIC INTOXICATION: MODULATION OF OXIDATIVE STRESS IN THE RAT HEART BY THE DIET
Autor/es:
FERRAMOLA ML, ANZULOVICH AC, GIMENEZ MS
Lugar:
Tucuman
Reunión:
Congreso; REUNION ANUAL DE LA SAIB; 2009
Institución organizadora:
Sociedad Argentina de Investigacion Bioquimica y Biologia Molecular
Resumen:
Cadmium chronic intoxication: Modulation of oxidative
stress in the rat heart by the diet.
Ferramola M, Anzulovich A.C., Giménez MS.
Laboratorio de nutrición y medio ambiente, IMIBIO-SL, CONICET,
UNSL.
E-mail: ferramolamariana@gmail.comferramolamariana@gmail.com
The aim of this study was to assess the effect of Cd2+ intoxication on
oxidative stress parameters in heart, and the putative protection of a
soy-based diet. Two months old male Wistar rats were separated into
six groups: 1, 3 and 5 were fed with a casein-based diet; 2, 4 and 6
were fed with a soy-based diet. Controls (1 and 2) received tap water;
groups 3 and 4 received tap water with Cd2+ 15ppm; groups 5 and 6,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
oxidative stress parameters in heart, and the putative protection of a
soy-based diet. Two months old male Wistar rats were separated into
six groups: 1, 3 and 5 were fed with a casein-based diet; 2, 4 and 6
were fed with a soy-based diet. Controls (1 and 2) received tap water;
groups 3 and 4 received tap water with Cd2+ 15ppm; groups 5 and 6,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
oxidative stress parameters in heart, and the putative protection of a
soy-based diet. Two months old male Wistar rats were separated into
six groups: 1, 3 and 5 were fed with a casein-based diet; 2, 4 and 6
were fed with a soy-based diet. Controls (1 and 2) received tap water;
groups 3 and 4 received tap water with Cd2+ 15ppm; groups 5 and 6,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
2+ intoxication on
oxidative stress parameters in heart, and the putative protection of a
soy-based diet. Two months old male Wistar rats were separated into
six groups: 1, 3 and 5 were fed with a casein-based diet; 2, 4 and 6
were fed with a soy-based diet. Controls (1 and 2) received tap water;
groups 3 and 4 received tap water with Cd2+ 15ppm; groups 5 and 6,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,
2+ 15ppm; groups 5 and 6,
tap water with Cd2+ 100ppm. Food and water were available ad libitum,2+ 100ppm. Food and water were available ad libitum,
during 4 months. TBARS levels and paraoxonase-1 (PON-1) activity in
serum, and antioxidant enzymes activities (SOD, CAT and GPx) in heart
were determined spectrophotometrically. TBARS levels and PON-1
activity increased in group #3, compared to #1, however TBARS
decreased in group #6, compared to #2 (p<0.05). CAT activity increased
in group #3 in comparison to #1, and it decreased in groups #4 and 6,
compared to #2 (p<0.05). No differences were found in GPx or SOD
activities.
In this study, we observed that Cd2+ intoxication increases oxidative
stress in serum and heart, modifying at the same time antioxidant
enzymes activities. There seems to be an induction of CAT activity in
the heart and PON-1 activity in serum with the lower concentration of
the metal. This, probably, could be due to a defense mechanism. No
increases of lipid peroxidation in serum were observed in the rats fed
with the soy-based diet.
stress in serum and heart, modifying at the same time antioxidant
enzymes activities. There seems to be an induction of CAT activity in
the heart and PON-1 activity in serum with the lower concentration of
the metal. This, probably, could be due to a defense mechanism. No
increases of lipid peroxidation in serum were observed in the rats fed
with the soy-based diet.
stress in serum and heart, modifying at the same time antioxidant
enzymes activities. There seems to be an induction of CAT activity in
the heart and PON-1 activity in serum with the lower concentration of
the metal. This, probably, could be due to a defense mechanism. No
increases of lipid peroxidation in serum were observed in the rats fed
with the soy-based diet.
2+ intoxication increases oxidative
stress in serum and heart, modifying at the same time antioxidant
enzymes activities. There seems to be an induction of CAT activity in
the heart and PON-1 activity in serum with the lower concentration of
the metal. This, probably, could be due to a defense mechanism. No
increases of lipid peroxidation in serum were observed in the rats fed
with the soy-based diet.