Determination of Cadmium-Induced Inflammatory Parameters in Wistar Rat

BIAGGIO V.,RUSSO, M. G., PEREZ DIAZ, M.,SANTILLAN, L. ANTON, R.,AND GIMENEZ MS

Tucuman

Congreso; Reunion Anual de la Soc. Argentina de Investigacion Bioquimica y Biologia Molecular; 2009

Sociedad Argentina de Investigacion Bioquimica

Determination of Cadmium-Induced Inflammatory Parameters in Wistar Rat Cerebral Cortex BIAGGIO V.,RUSSO, M. G., PEREZ DIAZ, M.,SANTILLAN, L. ANTON, R., GIMENEZ, M. S. Catedra de Bioquimica Molecular, FQByF, UNSL, IMIBIO-SL CONICET. e-mail: marcosgrusso@hotmail.commarcosgrusso@hotmail.com It has been observed that cadmium causes changes in the level of expression of genes involved in inflammatory disorders in different types cell in cerebral cortex. In this work we measured the concentration of cadmium in samples of cerebral cortex, the expression of inflammatory (TNF-a, VCAM-1 and iNOS) antiinflammatory parameters (PPAR-ã and MT III) and parameters oxidative stress (TBARS and NO). Our experimental model consisted of adult-male Wistar rats subjected to 15 ppm of cadmium in drinking water (Cd group) and without it (group CO). The total RNA was extracted by the method of TRIzol and levels of RNAm quantified using RT-PCR, the TBARS were quantified and the levels of NO using the reaction of Gries. We observed a significant increase in the concentration of cadmium in cerebral cortex (p<0.00001), the expression of inflammatory genes increased significantly in Cd group compared con el control group (TNF-á p: 0.0168; VCAM-1 p: 0.0053; iNOS: p: 0.0244) as well as that of MT III p: 0.0356. The expression of PPAR-ã did not change in Cd group. Moreover there was an increase in the levels of TBARS (p<0.0001) and a significative decrease in levels of ON (p< 0.0001) due possibly to the formation of peroxynitrites. We conclude that cadmium is able to cross the blood-brain barrier, reaching the nervous parenchyma and trigger an inflammatory process at the level of the cerebral cortex. possibly to the formation of peroxynitrites. We conclude that cadmium is able to cross the blood-brain barrier, reaching the nervous parenchyma and trigger an inflammatory process at the level of the cerebral cortex. that of MT III p: 0.0356. The expression of PPAR-ã did not change in Cd group. Moreover there was an increase in the levels of TBARS (p<0.0001) and a significative decrease in levels of ON (p< 0.0001) due possibly to the formation of peroxynitrites. We conclude that cadmium is able to cross the blood-brain barrier, reaching the nervous parenchyma and trigger an inflammatory process at the level of the cerebral cortex. possibly to the formation of peroxynitrites. We conclude that cadmium is able to cross the blood-brain barrier, reaching the nervous parenchyma and trigger an inflammatory process at the level of the cerebral cortex. p: 0.0168; VCAM-1 p: 0.0053; iNOS: p: 0.0244) as well as that of MT III p: 0.0356. The expression of PPAR-ã did not change in Cd group. Moreover there was an increase in the levels of TBARS (p<0.0001) and a significative decrease in levels of ON (p< 0.0001) due possibly to the formation of peroxynitrites. We conclude that cadmium is able to cross the blood-brain barrier, reaching the nervous parenchyma and trigger an inflammatory process at the level of the cerebral cortex. possibly to the formation of peroxynitrites. We conclude that cadmium is able to cross the blood-brain barrier, reaching the nervous parenchyma and trigger an inflammatory process at the level of the cerebral cortex. (p<0.0001) and a significative decrease in levels of ON (p< 0.0001) due possibly to the formation of peroxynitrites. We conclude that cadmium is able to cross the blood-brain barrier, reaching the nervous parenchyma and trigger an inflammatory process at the level of the cerebral cortex.