OY-BASED DIET MODIFIES THE INFILTRATION PROCESS IN CADMIUM INTOXICATED LUNGS

BOLDRINI GG; CIMINARI ME; GÓMEZ NN; MARTIN MOLINERO GD; BIAGGIO VS; SALINAS E; ÁLVAREZ SM

Mar del Plata

Congreso; Reunion Conjunta de Sociedades de Biociencias; 2019

SAIC

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on hematological parameters and bronchoalveolar lavages (BAL) under different diets due to the extensive infiltration we previously observed on lungs by optical microscopy. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots received soybean (Soy) as protein sources. Within each group: 1 lot received regular water (control-Co) and the other 15ppm of Cd in drinking water for 60 days. Blood samples were collected and they were analyzed using a hemocytometer ADVIA 360 (SIEMENS) and autoanalyzer A15 (BIOSYSTEMS). BAL was performed, cells were counted using a Neubauer?s chamber and LDH activity was measured. Total RNA was isolated from lungs using Trizol and cDNA was obtained: VCAM-1 and Nrf2 were determined by PCR, using S28 as control. ANOVA test was used for statistical analysis.White blood cells decreased in both intoxicated groups (p